Figure 4.

MMP inhibitor decreases migration of PMNs through collagen. Activated PMNs were treated with a MMP inhibitor (GM6001; 25 μM). Migration of WT PMNs treated with the MMP inhibitor (GM6001) and WT PMNs (not treated with the MMP inhibitor) across rat-tail collagen type 1 gels in vitro was compared, as detailed in Materials and Methods. PMNs that migrated through the collagen gels onto the 0.4-μm polycarbonate membrane (113 mm² surface area) were counted under a fluorescent microscope at 4 h and 24 h. Analysis of the data from three experiments (expressed as mean±sem) showed significantly decreased migration of WT PMNs treated with the MMP inhibitor (WT+I) when compared with the migration of WT PMNs not treated with the MMP inhibitor (WT) at 4 h (*, P=0.01) and 24 h (#, P=0.01).