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. 2009 Oct 2;87(1):69–77. doi: 10.1189/jlb.0509361

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Figure 5. — EGCG-treated collagen is resistant to cleavage by MMP-8. EGCG-treated collagen was analyzed for cleavage resistance to MMP-8 by protein gel electrophoresis. Lane M, Marker; Lane 1, collagen not treated with EGCG and MMP-8; Lane 2, collagen not treated with EGCG but treated with MMP-8; Lane 3, collagen treated with 0.5 mM EGCG but not with MMP-8; Lane 4, collagen treated with 0.5 mM EGCG and MMP-8; Lane 5, collagen treated with 0.75 mM EGCG but not with MMP-8; Lane 6, collagen treated with 0.75 mM EGCG and MMP-8; Lane 7, collagen treated with 1 mM EGCG but not with MMP-8; Lane 8, collagen treated with 1 mM EGCG and MMP-8. Lane 2 shows prominent bands of collagen cleavage. Collagen treated with EGCG (at 0.75 mM and 1 mM; Lanes 6 and 8) showed no bands of cleavage after treatment with MMP-8.