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Secondary structure models of the wild type Glf and the mutant 2-RD5 predicated by HMMTOP. (A) Wild type Glf has 12 TMSs and both N- and C- termini are inside the cytoplasm. (B) Mutant 2-RD5 has 9 TMSs and N-terminus is on the outside of the cytoplasm, while C-terminus is on the inside of the cytoplasm.
