Figure 4.

Identification of MetAP2 residue that is covalently modified by fumagillin. (A) HPLC chromatogram of the chymotryptic peptides derived from MetAP2 bound by fluorescein–fumagillin conjugate in the presence (data not shown) and absence (A) of ovalicin. Peaks I and II, absent in the control samples, are indicated in A. (B) Results of Edman degradation of peak I and peak II in A. The single-letter amino acid code was used. X denotes a blank signal for the corresponding sequencing cycle. C* denotes a cysteine-S-acetamide derivative. The corresponding sequences in MetAP2 are shown below the peptide sequences. Residues that gave either no signal or modified signal in Edman sequencing are underlined.