Figure 4.

mHSF3a is translocated to the nucleus in response to heat shock. (A) Schematic representation of mHSF3a, mHSF3b, and mutants fused with GFP. A nuclear localization signal was mutated in GFP-m3a-NLSmu. (B) COS7 cells were transfected with expression vectors. Cell extract was prepared and subjected to Western blotting using an antibody for GFP. (C) COS7 cells transfected with expression vectors for 48 h were treated with heat shock at 42°C for 1 h and then fixed with 4% paraformaldehyde. Fluorescence was examined under an Axiovert microscope.