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Sec3 localizes to centrioles. (A and B) Polarized MDCK cells were colabeled with anti-Sec3CT and either an anti-γ-tubulin (A) or an anti-kendrin (B) antibody. xy confocal sections are shown. Endogenous Sec3 was localized at kendrin- and γ-tubulin-positive structures. Bar, 20 μm.