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. 2010 Jan 1;21(1):18–28. doi: 10.1091/mbc.E09-01-0063

Figure 5.

Figure 5.

EM analysis of nuclei in multinucleated hyphae. (A) Overlay of a DIC and a fluorescence image of a young A. gossypii mycelium that was stained with Hoechst to visualize nuclei. Such mycelia with five to 10 tips and 50–100 nuclei were prepared for thin section EM analysis as described in Materials and Methods. Bar, 5 μm. (B–E) EM of nuclei in different nuclear cycle stages. The continuous nuclear membrane and nuclear pore complexes within the nuclear envelope can be seen in all images. Bars, 200 nm. (B) A single SPB (asterisk) is embedded in the nuclear envelope. A higher magnification is shown in the top right corner. (C) Duplicated SPBs (asterisks) connected by a bridge are embedded in the nuclear envelope. A higher magnification is presented in the top right corner. (D) A nucleus with spindle microtubules (arrow) and continuous nuclear membrane. The two SPBs were observed in the adjacent sections at positions marked by the asterisks. Magnifications are shown in the top and bottom right corners. (E) Montage of three EM images showing a nucleus in anaphase. The continuous nuclear envelope and spindle microtubules (arrow) are visible. The SPBs are in other sections.