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. 2010 Jan 1;21(1):18–28. doi: 10.1091/mbc.E09-01-0063

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Figure 6. — High-resolution EM analysis of A. gossypii SPBs and microtubules. (A) Electron micrograph showing five discrete SPB layers: the inner plaque (IP), central plaque (CP), intermediate layer 2 (IL2), intermediate layer 1 (IL1) and outer plaque (OP). Hook-like appendages (asterisk) extending from the CP anchor the SPB in the nuclear envelope (NE). Nuclear microtubules (nMTs) emanate from the IP and cMTs (cMTs) from the OP. (B) Two types of cMTs, one extending from the OP in a perpendicular direction and the other in a tangential direction. (C) Three serial sections of a duplicated SPB (SPB1 and SPB2) connected by a bridge (BR). Again, perpendicular and tangential cMTs nucleate at OPs. (D) SPB with capped nuclear and cMTs (arrows). The rounded caps at the microtubule ends are associated with electron dense material. (E) Low- and (F) high-magnification image of a cMT that ends in a distinct flare at its plus end (arrow). Bars, 100 nm (A–F).