Figure 1.

HIF-1 protein levels increase when worms are exposed to H₂S. (A) A representative Western blot analysis shows HIF-1 protein increases when worms are exposed to 50 ppm H₂S or 0.5% O₂. The blots were stripped and reprobed with an anti-actin antibody to confirm equivalent amounts of total protein. The size markers are shown in kilodaltons. (B) Average increase in signal relative to control ± SD of three independent experiments. *p < 0.05 (Student's t test) compared with air-treated animals.