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. 1998 Dec 22;95(26):15189–15193. doi: 10.1073/pnas.95.26.15189

Figure 4.

Figure 4

Measurement of ATP hydrolysis. Anion exchange chromatograms from four different MJ0577 reactions processed as described in Materials and Methods are shown. The samples are: A, a reference sample containing 10 nmol each of AMP, ADP, and ATP; B, MJ0577 alone (4° C); C, MJ0577 alone (80° C); D, MJ0577 plus M. jannaschii extract (4° C); E, MJ0577 plus M. jannaschii extract (80° C). Migration positions of AMP, ADP, and ATP are indicated above sample A. The initial large peak is the result of residual phenol/chloroform/isoamyl alcohol during the nucleotide extraction step (see Materials and Methods).