Table 2.
Representative values of the affinity increase factors s1- s4 for the six-subunit model Shown are representative examples of values of s1-s4 for all different combinations of the variants that are consistent with our experimental data. In each case, the values shown in this table lay in the middle of the range of values that are consistent with the experimental data. Whenever possible the values for 25°C and 30°C were chosen to be similar. Left column defines variant combinations: 3- or 4-activation, fast of slow T286 phosphorylation, and two different values of 1/Kα4 (20 or 60 nM).
| Two-phospho | One-phospho | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| T [°C] | 25 | 30 | 25 | 30 | ||||||||
| s1 | s3 | s4 | s1 | s3 | s4 | s1 | s3 | s4 | s1 | s3 | s4 | |
| 3-act, Fast, 20 | 1 | 64 | 4 | 1 | 45.2 | 4 | 2.4 | 1.4 | 2 | 4.4 | 1 | 4 |
| 3-act, Fast, 60 | 1 | 64 | 1 | 1 | 64 | 1 | 2.8 | 2 | 1 | 2.8 | 2 | 1 |
| 3-act, Slow, 20 | 2.8 | 128 | 1 | 2.8 | 128 | 1 | 3.2 | 11.3 | 1 | 5.3 | 11.3 | 1 |
| 3-act, Slow, 60 | ||||||||||||
| 2-act, Fast, 20 | 1 | 5.6 | 40.2 | 1 | 6 | 32 | ||||||
| 2-act, Fast, 60 | 1 | 64 | 2 | 1 | 64 | 2 | ||||||
| 2-act, Slow, 20 | 1 | 64 | 1 | 1.7 | 64 | 1 | 2.5 | 16 | 8 | 1.4 | 8 | 8 |
| 2-act, Slow, 60 | 1.1 | 32 | 1 | 1.6 | 32 | 1 | ||||||