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Mapping the regions of U19/Eaf2 that interact with ELL. Mammalian two-hybrid assay was performed to map the regions of U19/Eaf2 that interact with ELL. Indicated regions of U19/Eaf2 were cloned into pM. The entire ELL open reading frame was cloned into pVP16. PC3 cells were co-transfected with the vectors and luciferase reporter vectors. Twenty-four hours after transfection, the cells were harvested, lysed, and luciferase activity was measured using a luminometer.
