| ddH2O | 1750-x-y μl |
| 10 × T4 ligase buffer | 200 μl |
| SfiI-digested VHs | x μl (~30 μg) |
| SfiI-digested pCom3bX | y μl (~90 μg) |
| T4 ligase (200 units/μl) | 50 μl |
| Total | 2000 μl |
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