| 10 × RT buffer | 10 μl |
| 2.5 mM MgCl2 | 20 μl |
| 0.1 M DTT | 10 μl |
| RNase OUT™ (40 u/μl) | 5 μl |
| Superscript ™ III RT (200 u/μl) | 5 μl |
| Total | 50 μl |
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