| ddH2O | 46-x-y μl |
| 2 × High Fidelity PCR Master | 50 μl |
| FR3 | x μl (~100 ng) |
| CDR3s | y μl (~120 ng) |
| FR3-F (25 μM) | 2 μl |
| H3-R (25 μM) | 2 μl |
| Total | 100 μl |
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