| ddH2O | 46-x-y μl |
| 2 × High Fidelity PCR Master | 50 μl |
| CDR1-CDR2 | x μl (~100 ng) |
| FR3-CDR3 | y μl (~100 ng) |
| H1-F (25 μM) | 2 μl |
| HISR | 2 μl |
| Total | 100 μl |
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