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. 2010 Jan;16(1):106–117. doi: 10.1261/rna.1894510

FIGURE 3.

FIGURE 3.

Comparison of the activities of L sshRNAs differing in 3′ overhang and stem length. (A) Overhang comparison. L sshRNAs against the same target region as si19-3 and SG142, but with or without a 3′ overhang were chemically synthesized and their ability to inhibit HCV IRES-dependent luciferase expression was compared in 293FT cells. (B) Stem length comparison. L sshRNAs (UU-loop) against the same target region as SG142 and si19-3, but with different stem lengths were compared for their inhibitory activity in 293FT cells. SG105 differs from SG142 in lacking a 3′-UU overhang. (C) SG119 and SG117 comparison in 293FT cells. SG119 has a 19-nt antisense strand directly linked with a 17-nt sense strand, probably forming a 16-bp duplex and 4-nt loop (UGCA). (D) Stem length comparison with SG119 derivatives. SG131 and SG132 have UU to connect the 3′ end of the antisense and the 5′ end of the sense strands. They were compared with SG119 for target knockdown in 293FT cells. si131 has two complementary strands 16 nt in length and UU overhangs at the 3′ ends. Values labeled “as/s” represent the nucleotide lengths of antisense and sense strands.