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Target mRNA cleavage in vivo. 5′-RACE–PCR amplification of samples with and without target-specific siRNA or sshRNA treatments was analyzed on 2% agarose gels. L, 25-bp ladder; L′, 100-bp ladder; target pSG154m alone (lane 1); SG105 (lane 2); SG119 (lane 3); SG68 (lane 4); si19-3 (lane 5); no transfection control (lane 6); and water control (lane 7).
