FIGURE 8.

IFN-responsive gene OAS1-induction by sshRNAs in human PBMCs. Synthetic sshRNAs (20 nM) were complexed with DOTAP (Roche) and transfected into freshly isolated human PBMC in triplicate. DOTAP alone, or DOTAP complexed with equivalent amounts of either T7 transcribed shRNA or polyI:C were used as controls. RNA was extracted from cells 24-h post-transfection and quantitative RT-PCR was performed to measure the level of GAPDH and OAS1. The RNA level of OAS1 was normalized to GAPDH and is shown relative to DOTAP alone.