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L sshRNA is active in monomer form. L sshRNAs were treated with or without heating (95°C for 4 min) and snap-cooling (ice/water bath for 20 min). (A) The presence of monomers and multimers was examined using native 10% polyacrylamide gel electrophoresis and SYBR Gold staining. (B) Comparison of inhibition of IRES-dependent luciferase expression with and without the heat–cool step.
