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. 2010 Jan;16(1):118–130. doi: 10.1261/rna.1901810

FIGURE 5.

FIGURE 5.

Serum stability of sshRNAs. sshRNAs with and without 2′-OMe modifications were incubated with 10% human serum (Sigma-Aldrich) for various times at 37°C. At each time point, an aliquot was taken out, mixed with gel loading buffer (Ambion), and immediately stored in −80°C. The samples were analyzed by 12% denaturing PAGE (12% polyacrylamide, 20% formamide, and 8 M urea) and were stained with SYBR Gold (Invitrogen).