FIGURE 6.

Type I IFN-responsive gene OAS1-induction by sshRNAs in human PBMCs. sshRNA with and without 2′-OMe modifications (20 nM) were transfected in triplicate into freshly purified human PBMCs. Equivalent amounts of T7 transcribed shRNA or poly(I:C) were transfected as positive controls. The transfection reagent DOTAP alone was used as negative control. The cells were harvested 24 h post-transfection, and the mRNAs of OAS1 and GAPDH were measured by quantitative RT-PCR. The relative level of OAS1 mRNA is shown, normalized to GAPDH.