FIGURE 6.

Effect of altering base-stacking at the junction of the terminal loop and the upper stem of the eALAS IRE. The base pairs 14/18 and 13/20 of the eALAS IRE were systematically flipped in the presence of A19 or G19. Equilibrium dissociation constants were normalized to that of eALAS IRE, presented as K_rel ± SEM for at least three determinations for each RNA. P-value_eALAS refers to the comparison of the RNA of interest to the long (65 nt) eALAS IRE. (N.S.) Not significant. Nucleotide changes relative to wild type are circled.