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Sequence and secondary structure of the E. coli thiM riboswitch, based on the structure given by Serganov et al. (2006). (Black boxes) Site-directed mutations examined in this study, (lines with arrowheads) connectivity. Noncanonical pairs are denoted with Leontis–Westhof (Leontis and Westhof 2001) symbols. (Dashed line) Tertiary stacking interaction.
