FIGURE 3.

Poly(A) signal-dependent pausing in vitro on immobilized templates. (A) The immobilized reporter DNAs drawn to scale. Transcription was carried out as for Figure 2. (B) Cassette analysis for washed TECs isolated using Reporter 1. The average and standard deviation for three independent experiments is shown. (C) Cassette analysis for washed TECs isolated using Reporters 2a and 2b (independently cloned, presumably identical plasmids; see Materials and Methods). The average and range for two independent experiments is shown for reporter 2a, and the average and standard deviation for three independent experiments is shown for reporter 2b. Note that four out of the five results obtained clustered around the value of 82%. We consider the one low value (the 63% for reporter 2a) to be an outlier.