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Younger neurons (6-7 days old) were not sensitive to MSG damage. At day 6-7, cultured mouse cortical neurons in 24 wells were treated with different concentrations of MSG for 12h. 50 µl sample of medium was withdrawn from each well for LDH measurement. Cells were then lysed with 0.5% Triton X-100. This treatment gives total LDH. Percentage of cell death is calculated by dividing the LDH value by the total value in each well. n = 8.
