Table 1.
Conditions for polymerase chain reaction–restriction fragment length polymorphism analysis of the survivin promoter
| Position and base change | Primers | Annealing Temperature (°C) | PCR products (bp) | Enzyme | Digested PCR products (bp) |
|---|---|---|---|---|---|
| -1547A/G (rs3764383) | FP 5'-GCCCGATGCATTTAAATAAAAGA-3' | 52 | 118 | HincII | AA: 118 AG: 118/96/22 |
| RP 5'-GCAGAGAGTGAATGTTAAAGTTAA-3' | GG: 96/22 | ||||
| -644C/T (rs8073903) | FP 5'-AGGTCGTGCAGTCAACGATGT-3' | 52 | 89 | StyI | TT: 89 CT: 89/66/23 |
| RP 5'-CAGACGGGCATGAAGGACCCATG-3' | CC: 66/23 | ||||
| -625C/G (rs8073069) | FP 5'-TGTTCATTTGTCCTTCATGCGC-3' | 61 | 125 | BstUI | CC: 125 CG: 125/104/21 |
| RP 5'-CCAGCCTAGGCAACAAGAGCAA-3' | GG: 104/21 | ||||
| -241C/T (rs17878467) | FP 5'-GATTACAGGCGTGAGCCACT-3' | 61 | 128 | HaeII | TT: 128 CT: 128/104/24 |
| RP 5'-GTGTGCCGGGAGTTGTAGTC-3' | CC: 104/24 | ||||
| -31G/C (rs9904341) | FP 5'-CGTTCTTTGAAAGCAGTCGAG-3' | 66 | 329 | EcoO109I | CC: 329 CG: |
| RP 5'-TGTAGAGATGCGGTGGTCCT-3' | 329/234/92 GG: 234/92 |
bp=base pair; FP=forward primer; RP=reverse primer.