Table 3.
Regulation of the homologous Mtb genes in intraphagosomal bacilli. These Mtb genes are homologues to the differentially regulated Msm proteins shown in Table 1. The mRNA relative abundances were determined for the bacilli that infected naïve and activated murine bone marrow macrophages for 4, 24, and 48 hours respectively [42].
| Mtb gene | Mtb gene description | In naïve macrophages | In activated macrophages | Homologous Msm protein |
Similarity (%) |
||||
|---|---|---|---|---|---|---|---|---|---|
| 4 hr | 24 hr | 48 hr | 4 hr | 24 hr | 48 hr | ||||
| (Mtb genes matched to the genes of the upregulated Msm proteins in Table 1) | |||||||||
| Rv3127 | hypothetical protein | 1.3 | 1.7 | 1.9 | 1.7 | 21.3 | 7.5 | MSMEG_5246 | 60.00 |
| acg | hypothetical protein | 0.9 | 1.3 | 1.6 | 1.3 | 19.7 | 9.6 | MSMEG_5246 | 65.24 |
| acr | 14 KD antigen | 0.6 | 1.2 | 1.4 | 0.8 | 17.1 | 11.6 | MSMEG_3932 | 78.32 |
| Rv3129 | hypothetical protein | 1.1 | 0.6 | 1.3 | 1.3 | 11.8 | 7.8 | MSMEG_5243 | 76.59 |
| TB31.7 | hypothetical protein | 1.1 | 1.4 | 1.4 | 1.1 | 9.2 | 5.2 | MSMEG_3945 | 60.49 |
| Rv2005c | hypothetical protein | 0.9 | 1.0 | 1.2 | 1.1 | 6.7 | 2.5 | MSMEG_3950 | 65.52 |
| Rv0893c | hypothetical protein | 1.4 | 1.6 | 1.8 | 2.6 | 2.6 | 2.5 | MSMEG_0614 | 71.19 |
| Rv2026c | hypothetical protein | 1.6 | 1.5 | 1.7 | 1.8 | 2.4 | 2.2 | MSMEG_3945 | 60.99 |
| Rv0725c | hypothetical protein | 1.7 | 2.0 | 2.3 | 2.1 | 2.4 | 2.6 | MSMEG_0614 | 61.36 |
| Rv3767c | hypothetical protein | 2.3 | 2.0 | 1.7 | 2.4 | 2.2 | 2.0 | MSMEG_0614 | 60.60 |
| fadD9 | acyl-CoA synthetase | 2.4 | 2.2 | 1.8 | 2.4 | 2.2 | 2.0 | MSMEG_2956 | 82.08 |
| nrp | peptide synthetase | 1.3 | 1.6 | 1.6 | 1.5 | 2.1 | 1.7 | MSMEG_2956 | 73.58 |
| Rv1062 | hypothetical protein | 1.2 | 1.4 | 1.8 | 1.5 | 2.1 | 1.8 | MSMEG_5285 | 72.93 |
| Rv3787c | hypothetical protein | 1.4 | 1.3 | 1.3 | 1.6 | 1.7 | 1.7 | MSMEG_0614 | 62.79 |
| Rv2781c | hypothetical protein | 1.1 | 1.6 | 1.7 | 1.2 | 1.4 | 2.3 | MSMEG_4971 | 60.42 |
| Rv1889c | hypothetical protein | 1.4 | 1.3 | 1.3 | 1.5 | 1.4 | 1.4 | MSMEG_0614 | 62.39 |
| Rv0281 | hypothetical protein | 0.7 | 1.2 | 1.7 | 0.7 | 1.3 | 1.7 | MSMEG_0614 | 74.08 |
| hemL | glutamate-1-semialdehyde 2,1-aminomutase | 1.0 | 0.9 | 1.1 | 1.2 | 1.1 | 1.1 | MSMEG_0969 | 85.42 |
| Rv2765 | hypothetical protein | 1.0 | 0.8 | 0.9 | 1.0 | 0.9 | 0.9 | MSMEG_2669 | 73.57 |
| adhC | alcohol dehydrogenase | 0.9 | 0.8 | 0.8 | 0.8 | 0.7 | 0.7 | MSMEG_1037 | 74.06 |
| (Mtb genes matched to the genes of the downregulated Msm proteins in Table 1) | |||||||||
| cspA | cold shock protein | 0.5 | 0.6 | 0.4 | 0.4 | 0.3 | 0.3 | MSMEG_6159 | 98.50 |
| atpD | ATP synthase beta chain | 0.6 | 0.4 | 0.4 | 0.5 | 0.3 | 0.3 | MSMEG_4936 | 98.50 |
| Rv0227c | hypothetical protein | 0.6 | 0.5 | 0.4 | 0.5 | 0.3 | 0.3 | MSMEG_0317 | 75.74 |
| fadD5 | probable fatty-acid CoA ligase | 0.5 | 0.5 | 0.5 | 0.6 | 0.5 | 0.5 | MSMEG_0131 | 84.58 |
| panB | 3-methyl-2-oxobutanoate hydroxymethyltransferase | 1.0 | 1.2 | 1.0 | 0.9 | 0.7 | 0.7 | MSMEG_4298 | 83.27 |
| rpsQ | 30s ribosomal protein s17 | 1.0 | 1.0 | 0.8 | 0.8 | 0.7 | 0.7 | MSMEG_1445 | 90.72 |
| moaC2 | probable MoaC-2 protein involved in molybdopterin synthesis | 0.9 | 1.1 | 1.1 | 0.8 | 0.7 | 0.8 | MSMEG_5703 | 71.89 |
| phoY1 | probable phosphate transport system regulatory protein | 0.9 | 0.8 | 0.7 | 0.9 | 0.8 | 0.7 | MSMEG_1605 | 69.40 |
| Rv0042c | hypothetical protein | 0.9 | 0.9 | 0.8 | 0.9 | 0.9 | 0.7 | MSMEG_6913 | 65.03 |
| Rv3259 | hypothetical protein | 1.1 | 0.9 | 1.0 | 1.0 | 1.1 | 1.0 | MSMEG_1832 | 92.08 |
| phoY2 | probable phosphate transport system regulatory protein | 1.0 | 1.4 | 1.2 | 1.0 | 1.2 | 1.1 | MSMEG_1605 | 76.05 |
| Rv0875c | hypothetical protein | 0.8 | 1.1 | 1.4 | 0.9 | 1.6 | 1.5 | MSMEG_5694 | 74.05 |
| ispF | hypothetical protein | 1.4 | 1.6 | 2.1 | 1.6 | 1.7 | 2.3 | MSMEG_6075 | 69.53 |
| Rv0785 | hypothetical protein | 1.2 | 1.7 | 1.9 | 1.4 | 2.2 | 1.8 | MSMEG_5835 | 82.95 |
| Rv2669 | hypothetical protein | 1.3 | 1.5 | 1.6 | 1.4 | 2.3 | 1.7 | MSMEG_2789 | 63.80 |