. Author manuscript; available in PMC: 2010 Mar 1.

Published in final edited form as: Plant Cell Rep. 2008 Dec 9;28(3):469–480. doi: 10.1007/s00299-008-0651-5

Table 3.

Gene silencing effect of heterodimeric amiRNAs.

Transgenic line	No. of independent transformants^*	No. of plants with mutant phenotypes			Efficiency (%)	Effectiveness^€ (%)
Transgenic line	No. of independent transformants^*	none^≠ (%)	Weak (%)	Strong (%)	Efficiency (%)	Effectiveness^€ (%)
amiR-calA	16	n.a.			n.a.	n.a.
amiR-ap1AcalA	17	1 (6)	16 (94)	0	94	0
amiR-ap1A^PcalA	15	1 (6.7)	9 (60)	5^§ (33.3)	93.3	100
amiR-ap1A^P/amiR-calA	31^$	10 (32)	9 (29)	12^† (39)	n.a.	n.a.

T1 or F1 plants were analyzed.

^≠

plants showing wild type phenotype

^€

percentage of plants with null mutant phenotype showing indeterminate meristems among plants scored for strong phenotype

Among segregating F1 progenies, only bar-resistant plants were scored. n.a.: not applicable

^§

All 5 plants with strong ap1cal phenotype produced indeterminate meristems.

^†

4 out of 12 plants with strong ap1cal phenotype produced indeterminate meristems.