Expression of AR and steroidogenic machinery in prostate cancer cells
A. RT-PCR (upper panel) and Western blot (lower panel) analysis of AR mRNA and protein in C33 (androgen dependent), C81 (androgen independent), and AR negative DU145, and PC3 prostate cancer cell lines. Ribosomal L19 and β-actin were used as controls in RT-PCR and Western blots, respectively.
B. RT-PCR analysis of steroidogenic enzymes and other proteins required for androgen biosynthesis in C33 and C81prostate carcinoma cells.
C. Western blotting for StAR, P450scc (upper panel), and CYP17 (lower panel) protein in C33, C81, DU145 and PC3, PrEC, and H295R cells.