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Orientation of pre-tRNA in the central cleft of P protein. RNA/protein crosslinks were formed by irradiating truncated pre-tRNA substrates (see Fig. 1B) bound to RNase P holoenzyme reconstituted by using AzP-labeled P protein with the following unique cysteine substitutions: A, F16C; B, F20C; C, V32C; D, Y34C; E, I86C; F, V26C, G, A27C; and H, S102C.
