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. 1998 Dec 22;95(26):15212–15217. doi: 10.1073/pnas.95.26.15212

Figure 4.

Figure 4

Primer extension to map the crosslink sites in pTR14. Lanes G, A, T, and C represent dideoxynucleotide-terminated sequencing reactions by using unmodified pTR14 RNA as template. Lane 1, pTR14 crosslinked to holoenzyme reconstituted with AzP-S49C P protein; lane 2, pTR14 crosslinked to holoenzyme reconstituted with AzP-V26C P protein; and lane 3, uncrosslinked pTR14. The primer extension products are separated on an 8% polyacrylamide-urea gel. The corresponding nucleotide sequence of the pre-tRNA leader is shown. NT and CS represent the nucleotides in the cleft and cleavage site, respectively.