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. 2009 Oct 8;85(3):514–519. doi: 10.1093/cvr/cvp337

Figure 3.

Figure 3

KLF2 induction by resveratrol is ERK5 and AMPK independent, but partly dependent on MEF2. (A) Western blot of phospho-ERK5, ERK5, and house keeping protein alpha-tubulin from HUVEC stimulated for 8 h with ethanol vehicle, resveratrol (100 µM) or simvastatin (1 µM). (B) Effect of ERK5 silencing on resveratrol-induced KLF2 expression in HUVEC (*P < 0.05; n = 3; ANOVA, Tukey's HSD test). Insert shows representative western blot demonstrating ERK5 silencing efficiency. (C) KLF2 mRNA expression in HUVEC infected with MEF2ASA or GFP-control virus and stimulated with resveratrol or its vehicle (*P < 0.05; n = 3; ANOVA, Tukey's HSD test). (D) Effect of AMPK inhibition on resveratrol-mediated KLF2 expression in HUVEC (*P < 0.05; n = 3; ANOVA, Tukey's HSD test). Data expressed as mean ± SEM.