Results of spot detection on Nishahara and Champion study for (a) SameSpots, (b) SameSpots with PG spot detection, (c) SameSpots with manual editing, and (d) Pinnacle. For (a)-(c), the image is from gel 22; spots are marked by their estimated boundaries. For (d), the image is the average gel used for spot detection; pinnacles for detected spots are marked by a circled x.