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. 2009 Aug 14;81(6):1182–1188. doi: 10.1095/biolreprod.109.079418

FIG. 2.

FIG. 2.

Human MUC1 expression in early pregnancy series in human MUC1 transgenics. Endometrial extracts from Days 1, 3, and 4.5 postcoitum (D1, D3, and D4.5, respectively) from both wild-type and human MUC1 transgenics were analyzed by Western blotting as described in Materials and Methods. Human MUC1 detection was with either 214D4 (A) or HMFG-1 (B) monoclonal antibodies specific for human MUC1. No signal was observed for either antibody in extracts from wild-type mice. Expression of human MUC1 is reduced, but it persists during early pregnancy. C) Detection of human and mouse MUC1 by CT-1 antibody. Almost complete loss of MUC1 expression is detected in the wild type, whereas MUC1 expression persists at Day 4.5 in the transgenics, reflecting the presence of the human MUC1. D) A longer exposure of samples shown in C. E) Blots were reprobed with antibody specific for mouse cytokeratin 18/19 (KRT 18/19), epithelial cell markers serving both as a load control and to control for changes in total epithelial cell populations during pregnancy. Total protein extract from HES cells was used a positive control. Bar graphs in A′, B′, and C′ represent densitometric analyses to quantify MUC1 expression normalized to KRT18 of the above data provided as mean ± SD values in each case. *P < 0.05 relative to human MUC1 Day 1; ***P < 0.001 relative to human MUC1 Day 1.