Human MUC1 expression in interimplantation sites on Day 5 of pregnancy. Sections of Day 5 mouse uterine interimplantation sites from wild-type and human MUC1 transgenics were analyzed by indirect immunofluorescence with anti-CT-1 (red), anti-214D4 (green), and draq5 (arbitrarily assigned the color blue) as described in Materials and Methods. Sections of Day 5 wild-type uteri (A–D) were used as controls to compare to human MUC1 transgenic uterine sections (E–L). Human MUC1 expression persists in the luminal (E, F, and H) and glandular (I, J, and L) epithelia (indicated by arrows) at the interimplantation sites compared with mouse MUC1 expression (A, B, and D). Bar = 100 μm.