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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Aging Cell. 2009 Apr 21;8(4):353–369. doi: 10.1111/j.1474-9726.2009.00469.x

Figure 2.

Figure 2

Chronologically aging yeast do not exhibit senescence prior to cell death. CLS in synthetic dextrose minimal medium was determined using two measures of cell viability: colony forming units (CFU) (panel A) and staining with the vital dye FUN-1 (panel B). Yeast strains competent (WT, atg11Δ) or deficient (atg1Δ, atg7Δ) in autophagy were analyzed. Viability is expressed in terms of the percent of viability on day 1 and is plotted on a linear scale over a 5-day time period. FUN-1 staining was done as described in Materials and Methods. Cell density (OD600) measurements are shown (panel C).