Table 2 .

Composition of synthetic dextrose (SD) minimal and synthetic complete (SC) media.

Component*	SD	SC1	SC2
D-glucose	20 g/L	20 g/L	20 g/L
Ammonium sulfate	5	5	5
Yeast nitrogen base (-AS/-AA)	1.7	1.7	1.7
Essential
L-Histidine	20 mg/L	20 mg/L	73 mg/L
L-Leucine	30	30	367
L-Lysine	30	30	73
Uracil	20	20	73
Non-essential
Adenine		20	18
L-Alanine			73
L-Arginine		20	73
L-Asparagine			73
L-Aspartic acid		100	73
L-Cysteine			73
L-Glutamine			73
L-Glutamic acid		100	73
L-Glycine			73
Inositol			73
L-isoleucine		30	73
L-Methionine		20	73
p-Amino benzoic acid			73
L-Phenylalanine		50	73
L-Proline			73
L-Serine		400	73
L-Threonine		200	73
L-Tryptophan		20	73
L-Tyrosine		30	73
L-Valine		150	73

^*Concentrations of glucose, ammonium sulfate, and yeast nitrogen base are listed in g/L. Concentrations of other supplements are listed in mg/L. Yeast nitrogen base does not contain ammonium sulfate (AS) or amino acids (AA). SD and SC1 are prepared according to Sherman Sherman, 2002). SC2 is prepared according to Fink Amberg et al., 2005;Styles, 2002). In some experiments, as indicated in the text, different combinations and/or amounts of supplements were present in synthetic minimal media at concentrations equal to, or three-fold higher than, those shown in the SC1 column.