Table 1. Primer sequences and PCR conditions.
Exon | Sequence of primers | Number of cycles | Annealing temperature (°C) |
---|---|---|---|
2 |
F-AGTCTCAGCCATCTCCTCGC |
35 |
62 |
R-TGGCCTGTCTGGAGCCTG | |||
3 |
F-GGGACAGTCTCAGCCATCTC |
35 |
60 |
R-CAGCTCCTCGTAGTCCTCC | |||
4 |
F-AGAAAGCTGGAGGAGCCG |
35 |
60 |
R-GCGGCAGCCCTGTCTGTAC | |||
5 |
F-GGGGCAGGTGGTGTTCAGA |
35 |
60 |
R-GGCAGCCTCACCAGCCTAG | |||
6 |
F-GGGCAGGTGGTGTTCAGA |
35 |
60 |
R-CCTTGGTCCTTCTAGCCTCAG | |||
7 |
F-CATCCTGATTTCAGGGTTCC |
35 |
60 |
R-CTCTGGCCATGCCTCCAG | |||
8 |
F-AGCTGAGGTTTAAAGGGGGA |
35 |
60 |
R-TCTCTTTGGGTCCACTTTGG | |||
9 |
F-ACATACAAGGTCCTGCCTGG |
35 |
60 |
R-GCATTAACTAGTGCTATTCTAAGTTCC | |||
10 A |
F-GGTGTTGGTCCTTTGTCCAC |
35 |
60 |
R-CTCTGGCATATCCGTCAGGT | |||
10 B |
F-CTTCAAGTCTGCCCCACTGT |
35 |
60 |
R-TAGGCTCAGAGCAAGGGAAG | |||
11 | F-CATTTTGGTATTTGAAATGAAGG |
35 | 60 |
R-CCATTTGATTCAGGCTGTTG |