Table 2. Primers used for polymerase chain reaction amplification and sequencing of BMP4, VSX2, CRYBA4, GDF6, OTX2, RAX, SIX3, SIX6, SOX2, and LRP6.

Gene	Exon	Primer sequence (5'-3')	Amplicon size (bp)	Annealing temperature (°C)
BMP4	3-F	CCATCTTGCCCCTCCATTTCTA	570	65
	3-R	CTTCTTCCCCAGGGCTTTCACT
	4a-F	TGCTTATTTTCCCCCAGTAGGT	704	62
	4a-R	GGCGCCGGCAGTTCTTATTCTT
	4b-F	GGGCCAGCATGTCAGGATTAGC	575	62
	4b-R	TGTGGGTGAGTGGATGGGAACG
	RFLP-F	CGGGAGAAGCAGCCAAACTATG	237	65
	RFLP-R	CTTCTTCCTGGCCCGCTGTGAG
VSX2	1-F	AGGGGGCACCTGGGACCAAC	586	72
	1-R	CCCGGCCTGGCAGGAACTTT
	2-F	CGGCGCGGGAGAGGTCAG	427	66
	2-R	GCAGATTCCGCCAAACAAC
	3-F	TGCCCAGGAGACACAGAGG	347	64
	3-R	ACATGATCATAGGCAAGCACA
	4-F	AGGACGCCCTGCTGGAGAAA	380	68
	4-R	CCAAGTGCCCCTGCCTCAA
	5-F	CTGCGGTGTGGGGAGTAAG	574	65
	5-R	GTGGGGAACAGGGAGGATG
CRYBA4	2-F	ACTCCTGGACTCCCTATGTG	319	60
	2-R	ATTCAACCTCCCTGTATGTG
	3-F	TCTTGCCTTCCTGGCTCCTG	430	62
	3-R	TGCGCAACCTGCATAATCTT
	4-F	CCCCTGAATGGTTGTGACT	396	62
	4-R	AACCGAGGCTTGGAGAGGAA
	5-F	AAGGGCAAATGGCAAGGTT	415	62
	5-R	TGGGCATCAGAGCACAAAAG
GDF6	1-F	GGCGGGGCCGGGGTTTGT	635	72
	1-R	TAGCCTCCAGCGGGAACAGC
	2a-F	CGGCCGACCTGCCCCCACTC	504	70
	2a-R	CCGGCCGAAGCCCAGACTCC
	2b-F	GCCGGCCGGCTGGGAAGTCT	557	70
	2b-R	GCGAGCGCAGCGGGAAGTCG
	2c-F	GCGCACGGCCTTCGCCAGTC	469	70
	2c-R	CCAGCGCCAGCTTCCTCCTC
OTX2	3-F	TTTGCTTTGCCCTTAGTTCC	424	62
	3-R	CCCTGTTCTCTGCTTGGTCA
	4-F	ACGGTGGGGAGAGCATTGGT	445	62
	4-R	CCTGGCCCCTTAGTGAGTGA
	5a-F	CTGCCCATGTAGGATAGATT	440	61
	5a-R	ATGCCCCCAAAGTAGGAAGT
	5b-F	GCTTCCATCTCCCCACTGTC	562	61
	5b-R	GGCCCTTCGTTTTTCCTTCT
RAX	1-F	TTCGCCCGCGGAGCTTGACCT	527	66
	1-R	CCCCAACCCCGCGCCCAGTT
	2-F	CCATCGCCGCCCTCACCA	522	66
	2-R	ACTCTGGGCATGCCAAGTCG
	3-F	TTGAGGGGGACGGAGTGGAG	716	69
	3-R	GCAGGCGACAGGGAAAGAGG
SIX3	1a-F	TCATCGCCCCTCTCCTCCTCTT	398	70
	1a-R	GCCGCTCGATGTCGCCCGTCTC
	1b-F	CGGCGGCGGCGGCTCCAG	387	72
	1b-R	CGCACGCGGTACTTGTCCAC
	1c-F	GCGTGCGAGGCCATCAACAA	552	65
	1c-R	CACGGCTTCCCTGGCTCTCA
	2-F	GCTCGGGTTCTGCCTCTC	457	64
	2-R	TCGGTTTGTTCTGGGGATGG
SIX6	1a-F	TGTGTCCCGCTGCCCCAATC	514	65
	1a-R	TTCTGTTCGCCGTCCCAAATG
	1b-F	CCTTTCACGGTGGCAACTAC	514	65
	1b-R	GACAGACCGCGCTCCCAACTC
	2-F	CGCCTTGCCGAGTAATCCT	447	70
	2-R	AGCCCGCGGGTCCCTGGTCAC
	HA-SSCP-F	TCGCCTTAACTGCTGGGGTCTT	253	67
	HA-SSCP-R	AGTGGCCGCCTTGCTGGATA
SOX2	1-F	CGCCTCCCCTCCTCCTCTC	443	68
	1-R	CGCCGGGGCCGGTATTTAT
	2-F	GGGCGCCGAGTGGAAACTT	473	65
	2-R	GGGTGCCCTGCTGCGAGTA
	3-F	CACGGCGCAGCGCAGATGC	459	65
	3-R	TTTGCACCCCTCCCATTTC
LRP6	1-F	CTCCTCGCCTCCCCCACTTCTG	279	72
	1-R	CTGCTCCCGGGCCCCTTTCTCT
	2-F	ATTTTCGACAGTCTTTGCTCAC	589	65
	2-R	TTCTTTTCTCATAGGGGTCAGG
	3-F	GCGCGGCCTGAGCTTTCTTTA	410	64
	3-R	CTTCTTCCCCTCTGGCACTTAG
	4-F	ATTTTAATGGGAGAGGTGACG	395	60
	4-R	TTTATTCCCGCCAACTATCTTT
	5-F	AATTTTGGCTTATCACAGTT	330	58
	5-R	GGTCTCCCAAAGCAGTAT
	6-F	TTTTATATTTATTTTTCAGTTC	575	50
	6-R	ATGTTATCTTAGTCAATGTTTT
	7-F	GGGATGGATCTCACCTTTAG	478	58
	7-R	GATCAGCAGCCATTTCTCA
	8-F	GGGGGAAAAGTGGTCAAA	538	56
	8-R	GGGGGCAGTAAAGAAGGT
	9-F	TGGGAGCAAGACATAATCATAG	690	64
	9-R	TGGCACGCACCTGTAGTCCT
	10-F	GGATCCTCTTGCCCCTGACA	560	62
	10-R	TAACCCATTCCCCTCTTTCTTC
	11-F	ATTGTAGCCGTGATTTTGTTTA	577	58
	11-R	TCAGGAGTATCTAGGGAGTTAT
	12-F	AAGCATGGGGTCAGAAGATAGA	777	62
	12-R	AAAGTGCTGGGTTACGGACATA
	13-F	TGAGGGCATGCCAAAGAAT	499	58
	13-R	AATAAGCTACCAGGTCCAGAAT
	14-F	GTGTGCCCATGTAGGTGTAAGC	511	64
	14-R	TAGTGGCCCAGGAAAGAAAGTC
	15-F	CCGCCTCAGTCTCCCAAAGT	491	68
	15-R	TGCCAAGAAATGTGCCAAAAAC
	16-F	TATCTAGTTTATTGGCTGTT	506	52
	16-R	CTAAAAGTGCATGAAAGTCT
	17-F	AAGCTGATTATACATTTGATTT	403	64
	17-R	GGGCAGGGTGGCAGAGAA
	18-F	TAAAGGAAGTAATGTGAAAACC	521	58
	18-R	TGAAAAACCCCAACTGAC
	19-F	AGGCACCTTTTGATTCTTG	495	61
	19-R	CGCCCGGCTGATTTCTATGTAT
	20-F	TTCAGGGCGTGGTATGTATGT	578	58
	20-R	TATCTAAGGCCTTCTGTGTAAA
	21-F	AGCTATTCTTGGCCTTGTTCTA	508	61
	21-R	AGTCCTTTGAGCCTTTTATGC
	22-F	TTTTAGCCATGATGAGGTCTTA	373	64
	22-R	GGGGCTATATCAGGTCCACAAC
	23-F	GAAAATTGCCTCTTGGTCTGTG	550	65
	23-R	TGGTCTGCCTCATCCTTCTCTA