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. 1998 Dec 22;95(26):15218–15222. doi: 10.1073/pnas.95.26.15218

Figure 3.

Figure 3

Transcription reactions using discrete topoisomer pools in the reconstituted Sulfolobus system. Transcription reactions using T6 topoisomer pools, at 75°C (a) or 48°C (e). The specific linking difference (σ) values, calculated as described in Materials and Methods, are given under the appropriate lanes. (b and f) Transcription reactions using 16S pools at 75°C and 48°C as indicated. (c and d) Effect of substituting adenosine 5′-[β,γ-imido]triphosphate (AMP-PNP) for ATP in the reconstituted transcription reaction on negatively supercoiled (σ = −0.042) T6 template (c) and positively supercoiled (σ = +0.056) T6 template (d) at 75°C. Lanes 1–3 contain 100, 50, and 25 μM ATP, respectively, together with 100 μM GTP, CTP, and UTP. Lanes 4–6 contain 100, 50, and 25 μM AMP-PNP, respectively, together with 100 μM GTP, CTP, and UTP.