Skip to main content
. 1998 Dec 22;95(26):15218–15222. doi: 10.1073/pnas.95.26.15218

Figure 5.

Figure 5

Single-round transcription assays. (a) Establishment of single-round assay. Transcription of negatively supercoiled 16S promoter was conducted at 75°C with various orders of addition of heparin and NTPs, as indicated. (b) Linear (lanes 1, 3, 5, and 7) or supercoiled (lanes 2, 4, 6, and 8) template was preincubated with TBP, TFB, and RNAP in the presence of ATP, CTP, and GTP at 48°C (lanes 1–4) or 75°C (lanes 5–8). Heparin was added to prevent reinitiation, and reactions were incubated at 48°C (lanes 1, 2, 5, and 6) or 75°C (lanes 3, 4, 7, and 8) for an additional 5 min.