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Sensitivity of YFP mutants to chloride at pH 7.5.
| Mutant | EC50 (mM) |
|---|---|
| H148Q | 106a–197 |
| H148Q/I152L | 85a–88 |
| H148Q/V150A/I152L | 61 |
| H148Q/V163S | 39a–62 |
| H148Q/V163L | 77 |
| H148Q/V163T/F165Y | 55 |
| H148Q/V150S/V163T | 92 |
aPurified protein. EC50 was measured in bacterial lysate with YFP-H148Q containing additional single or double mutations. (Modified from Galietta et al., 2001a).
