Table 1.

RTK analysis of NF1-related MPNSTs

	Protein Analysis									DNA Analysis						Ligands
	PDGFRA			PDGFRB			EGFR			PDGFRA		PDGFRB		EGFR
Case	IHC	WB Exp	WB P	IHC	WB Exp	WB P	IHC	WB Exp	WB P	FISH	Mut	FISH	Mut	FISH	Mut	PDGFA	PDGFB	TGF-α
1a	pos	NE	NE	pos	NE	NE	High	NE	NE	D	NE	D	NE	A	NE	+	+	+
1bb	pos	+	+	pos	+	++	High	++	++	D	wt	T	wt	LP	wt	+	+	+
2b	pos	+	−	pos	+++	++	Intermediate	NE	NE	A	wt	HP	wt	LP	wt	+	+	+
3b	pos	NE	NE	pos	NE	NE	High	NE	NE	D	wt	T	wt	T	wt	NE	NE	NE
4	pos	NE	NE	pos	NE	NE	High	NE	NE	D	wt	D	Glu485Lysa	A	wt	NE	NE	NE
5b	pos	NE	NE	pos	+	+	High	+++	+++	HP	wt	T	wt	HP	wt	NE	NE	NE
6a	High	+	+	High	+	+	Low	+	+	LP	wt	HP	wt	HP	wt	+	+	+
6cc	High	NE	NE	pos	NE	NE	Intermediate	NE	NE	LP	wt	HP	wt	HP	wt	NE	NE	NE
7	pos	++	++	pos	++	++	High	++	++	LP	wt	T	nv	HP	wt	+	+	+
8b	pos	+	+	pos	NE	NE	Low	+++	+++	D	wt	D	wt	LP	wt	+	+	+
9	pos	NE	NE	pos	NE	NE	neg	NE	NE	D	wt	D	NE	D	wt	NE	NE	NE
10	pos	++	++	pos	++	++	High	+++	+++	D	wt	T	Glu485Lysa	LP	wt	+	+	+
11	pos	NE	NE	pos	NE	NE	High	NE	NE	D	wt	D	wt	D	wt	+	+	+
12	pos	NE	NE	pos	NE	NE	Intermediate	NE	NE	D	wt	T	wt	D	wt	+	+	+
13	Low	++	++	neg	+	++	High	++	++	NE	NE	NE	NE	NE	NE	+	+	+
14	pos	NE	NE	pos	NE	NE	Low	NE	NE	NE	wt	NE	wt	NE	wt	+	+	+

Abbreviations: IHC, immunohistochemistry; WB Exp, receptor expression by Western blot; WB P, receptor phosphorylation by Western blot; FISH, fluorescence in situ hybridization; Mut, mutational status of receptors; PDGFRA, platelet-derived growth factor receptor A; PDGFRB, platelet-derived growth factor receptor B; TGF-α, transforming growth factor-a; pos, positive; neg, negative; +, level lower than in the positive control; ++, level similar to that in the positive control; +++, level higher than in the positive control; D, disomy; A, gene amplification; LP, low polysomy; HP, high polysomy; T, trisomy; NE, not evaluable; wt, wild type.

^aPolymorphism.

^bCases carrying p16 homozygous deletions.

^cCase carrying p16 promoter methylation.