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. 2010 Jan;38(1):100–107. doi: 10.1124/dmd.109.029025

Fig. 2.

Fig. 2.

Induction of CYP4F11 by cytokines through the JNK pathway. a, effects of cytokines and TPA on CYP4F11 transcripts in HaCaT cells. Cells were treated with 10 ng/ml TNF-α, 10 ng/ml IL-1β, or 100 ng/ml TPA for 24 h. Cells treated with 0.1% DMSO or 0.1% bovine serum albumin were used as the vehicle control. The expression of CYP4F11 was quantitated by qRT-PCR. Each data point represents n = 5. ∗, P < 0.05, ∗∗, P < 0.01 compared with the control group. b and c, effects of pathway inhibitors on the induction of CYP4F11 by cytokines. HaCaT cells preincubated with or without SP600125 (JNK inhibitor) or SB203580 (p38 inhibitor) were treated with 10 ng/ml TNF-α (b) or 10 ng/ml IL-1β (c). At 24 h post-treatment, total RNA was isolated, and the expression of CYP4F11 was quantitated by qRT-PCR. Cells preincubated with 0.1% DMSO and then treated with 0.1% bovine serum albumin were used as the vehicle control. Each data point represents n = 5. ∗∗, P < 0.01 compared with the relative control group. d, TPA had no effect on c-Jun phosphorylation in HaCaT cells. Cells were treated with 100 ng/ml TPA and collected at different time points as indicated in the figure. Whole cell lysate was isolated from each sample, and 20 μg of proteins from each sample was loaded for Western blot assay. β-Actin was used as the loading control. e, effects of TNF-α on c-Jun phosphorylation in HaCaT cells. Cells preincubated with or without 20 μM SP600125 were treated with 10 ng/ml TNF-α and collected at different time points as indicated in the figure. Whole cell lysate was isolated from each sample, and 15 μg of proteins from each sample was loaded for Western blot assay. β-Actin was used as the loading control. f, inhibition effects of SP600125, 9-cis-retinoic acid, and all-trans-retinoic acid on TNF-α-induced c-Jun phosphorylation in HaCaT cells. Cells preincubated with or without 20 μM SP600125, 1 μM 9-cis-retinoic acid, or 1 μM all-trans-retinoic acid were treated with 10 ng/ml TNF-α and collected at different time points as indicated in the figure. Whole cell lysate was isolated from each sample, and 15 μg of proteins from each sample was loaded for Western blot assay. β-Actin was used as the loading control.

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