Skip to main content
. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: J Immunol. 2009 Apr 15;182(8):4776–4783. doi: 10.4049/jimmunol.0800242

Figure 1. FRET detects clustering of Bap31-YFP.

Figure 1

Figure 1

Figure 1

FRET between Bap31-YFP and: A) Bap31-CFP, B) Bap29-CFP and C) HLA-A2-CFP. For all FRET pairs, FRET efficiency increases with increasing A:D ratio (YFP/CFP), a characteristic of FRET between specifically-associated donor and acceptor-tagged molecules. FRET between randomly distributed molecules is independent of A:D (18). The slopes of the plots indicate the FRET efficiency for each pair.