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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: J Immunol. 2009 Apr 15;182(8):4776–4783. doi: 10.4049/jimmunol.0800242

Figure 3. Fraction of ERGIC-53-positive pixels that are also positive for calnexin (Cxn) an authentic ER marker, Bap29 (B29) and Bap31 (B31).

Figure 3

Left bars – apparent fraction of ERGIC marker colocalizing with the three proteins at 37°C. Right bars- apparent fraction of ERGIC marker colocalizing with the three proteins at 15°C. The reduction in colocalization of ERGIC-53 with the other markers at 15°C reflects the temperature-induced block in cycling of ERGIC-53 between the ERGIC and the ER (27). Note that this block affects colocalization of ERGIC-53 and Bap31, much less than it affects the colocalization of ERGIC-53 with calnexin, an ER-resident protein. The result for ERGIC-53 and Bap29 is similar to that for ERGIC-53 and calnexin, which implies that Bap29, like calnexin, is restricted to the ER. The data are averages for 20 (15°C) and 35 (37°C) cells.