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. Author manuscript; available in PMC: 2010 Jan 6.
Published in final edited form as: Nat Cell Biol. 2001 Mar;3(3):267–275. doi: 10.1038/35060059

Figure 1. Inhibition of serine proteases during differentiation of 3T3-L1 cells reduces adipose conversion.

Figure 1

a , e–h, Staining of cells with Oil red O to visualize the extent of adipose conversion. b, i, Quantification of lipid accumulation; absorbance is designated as 1.0 for adipocytes. Data are means ± s.d. c, d, Western blotting of 3T3-L1 nuclear lysates to detect C/EBPβ (c) and PPARγ (d). Pre, 3T3-L1 preadipocytes; Adi, adipocytes, EcoWT, differentiating cells treated with WT ecotin; EcoRR, differentiating cells treated with EcoRR. Where indicated, adipocytes were treated with PAI-1 or α-2-antiplasmin (α2-AP).