Fig. 1.

Activation of hCAR1, hCAR3, and chimeric constructs in cell-based reporter assays. Schematic structure organization of the reference (hCAR1), splice variant (hCAR3), and chimeric human CAR transcripts (A). HepG2 cells were transfected with CYP2B6-PBREM (B) or CYP3A4 XREM (C) reporter construct in the presence of hCAR1, hCAR3, or hCAR chimeric expression vectors. Transfected cells were treated with vehicle control (0.1% DMSO) or 1 μM CITCO for 24 h. Luciferase activities were determined and expressed relative to hCAR3 vehicle control. Data represent the mean ± S.D. (n = 3) (*, p < 0.05; **, p < 0.01).