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. 2010 Jan;332(1):106–115. doi: 10.1124/jpet.109.159210

Table 1.

Generation of hCAR chimeric constructs

Vector Names Mutagenesis Primers Source Vector Introduced Amino Acids
hCAR1+YLT 5′-CATGGCCCTCTTCTCTCCTTATCTTACAGACCGACCTG-3′ pCR3/hCAR1 YLT (271–273)
5′-CAGGTCGGTCTGTAAGATAAGGAGAGAAGAGGGCCATG-3′
hCAR1+AP 5′-CTCTTCTCTCCTGCTCCCGACCGACCTGGAGTTACC-3′ pCR3/hCAR1 AP (271–272)
5′-GGTAACTCCAGGTCGGTCGGGAGCAGGAGAGAAGAG-3′
hCAR1+A 5′-CCCTCTTCTCTCCTGCTGACCGACCTGGAGTTAC-3′ pCR3/hCAR1 A (271)
5′-GTAACTCCAGGTCGGTCAGCAGGAGAGAAGAGGG-3′
hCAR1+P 5′-CCTCTTCTCTCCTCCCGACCGACCTGGAG-3′ pCR3/hCAR1 P (271)
5′-CTCCAGGTCGGTCGGGAGGAGAGAAGAGG-3′