Table 1.
Vector Names | Mutagenesis Primers | Source Vector | Introduced Amino Acids |
---|---|---|---|
hCAR1+YLT | 5′-CATGGCCCTCTTCTCTCCTTATCTTACAGACCGACCTG-3′ | pCR3/hCAR1 | YLT (271–273) |
5′-CAGGTCGGTCTGTAAGATAAGGAGAGAAGAGGGCCATG-3′ | |||
hCAR1+AP | 5′-CTCTTCTCTCCTGCTCCCGACCGACCTGGAGTTACC-3′ | pCR3/hCAR1 | AP (271–272) |
5′-GGTAACTCCAGGTCGGTCGGGAGCAGGAGAGAAGAG-3′ | |||
hCAR1+A | 5′-CCCTCTTCTCTCCTGCTGACCGACCTGGAGTTAC-3′ | pCR3/hCAR1 | A (271) |
5′-GTAACTCCAGGTCGGTCAGCAGGAGAGAAGAGGG-3′ | |||
hCAR1+P | 5′-CCTCTTCTCTCCTCCCGACCGACCTGGAG-3′ | pCR3/hCAR1 | P (271) |
5′-CTCCAGGTCGGTCGGGAGGAGAGAAGAGG-3′ |